Fig. 3
A phase II clinical trial with DC pulsed with tumoral cell lysates from patients having follicular lymphoma. a Intradermal injection of 0.2 mL of DC-pulsed with tumoral lysates (5 × 106 DC/mL) (5 sites) and KLH intradermal injection (100 µg in 0.2 mL) used as a control. Magnification: ×1; b Delayed hypersensitivity after intradermal injections (1) and cutaneous biopsies (2) showing important CD3 positive infiltrate and CD68 positive macrophages; c [18F] fluorodeoxyglucose-positron emission tomography scanning (FDG pet-scan): (1) before vaccination with tumoral lymph node (yellow arrow), (2) 15 days after the vaccination with increased hypermetabolism of the lymph node (white arrow), (3) 2 months after the vaccination with the disappearance of the hypermetabolism (yellow arrow), suggesting an activation of the IECs within the lymph node. 1: injection site with 0.2 mL of DC-pulsed with tumoral lysates; 2: injection site of control; DC: dendritic cells; KLH: Keyhole limpet hemocyanin