Fig. 2
From: Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells
Role of MAPK signaling in mediating K-Ras-induced CD137 expression. CD137 mRNA levels in T-Rex/K-Ras (a), HCT116 (b), Panc-1 (c), and SW1990 cells (d) measured by real-time PCR. The cells were first transfected with 100 nmol/L control siRNA or siRNA against MAPK1 for 24 h, then incubated with doxycycline for 48 h. Data are presented as mean ± SEM of three repeated experiments. Statistical analysis: two-tailed unpaired t-test. *P < 0.05; ***P < 0.001. CD137 tumor necrosis factor receptor superfamily member 9, Doxy doxycycline, MAPK mitogen-activated protein kinase, siCTL control siRNA, siMAPK1 siRNA against MAPK1, PCR polymerase chain reaction