Skip to main content

Table 2 Advantages and disadvantages of several single cell-analytic methods

From: Immune contexture defined by single cell technology for prognosis prediction and immunotherapy guidance in cancer

Single cell analysis technology Advantages Disadvantages References
Single-cell mass cytometry Detect multiple protein markers simultaneously Providing only a coarse level of resolution [92, 93]
Single-cell RNA sequencing Characterize the transcriptomes of cell types in different tissues and identifying their transcriptional signatures Initial amounts of RNA obtained from a single cell are low
Exhibiting higher levels of noise and more zero values
[94, 96]
Single-cell proteomics Capture proteomic information from individual cells
Rich in information
Multicellular Single-cell proteomics with high sensitivity, specificity and low cost needs to be developed [97,98,99]