Table 2 Advantages and disadvantages of several single cell-analytic methods
| Single cell analysis technology | Advantages | Disadvantages | References |
|---|---|---|---|
| Single-cell mass cytometry | Detect multiple protein markers simultaneously | Providing only a coarse level of resolution | [92, 93] |
| Single-cell RNA sequencing | Characterize the transcriptomes of cell types in different tissues and identifying their transcriptional signatures |
Initial amounts of RNA obtained from a single cell are low Exhibiting higher levels of noise and more zero values | [94, 96] |
| Single-cell proteomics |
Capture proteomic information from individual cells Rich in information | Multicellular Single-cell proteomics with high sensitivity, specificity and low cost needs to be developed | [97,98,99] |