Fig. 2

Effects of DUXAP8 on PC proliferation and apoptosis in vitro. a Analysis of DUXAP8 expression levels in PC cell lines (AsPC-1, BxPC-3 and PANC-1) compared with a normal pancreatic cell line (HPDE6) by qPCR. Data are shown as fold change (2−ΔΔCT) and the mean ± SD from three independent experiments. b DUXAP8 knockdown in PC cells transfected with si-DUXAP8 1# or si-DUXAP8 2#. c MTT assays were performed to determine the cell viability of AsPC-1, BxPC-3 and PANC-1 cells after the transfection of siRNA against DUXAP8. d Representative results of the colony formation of PC cells transfected with the siRNA of DUXAP8. e Flow cytometry assays were performed to analysis the cell cycle progression when BxPC-3 and PANC-1 cells transfected with siRNA against DUXAP8. f Flow cytometry assays were performed to analysis the cell apoptotic in siRNA-transfected BxPC-3 and PANC-1 cells. Representative images and data based on three independent experiments. Bars: s.d, *P < 0.05, **P < 0.01