Fig. 5

Snail promotes NPC cell migration and invasion mostly by down-regulating TEL2. a–f Migration and invasion activity of indicated cells were measured at 24 h in transwell assays. The number of cells passing through the membrane in each well was analyzed in triplicate and repeated three times with similar results. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. b, d, f Representative images of transwell assays. Red scale bar, 100 μm. e, f The migration and invasion abilities of S26 cells with NC+scr, NC+shSnail or siTEL2+shSnail. The number of cells passing through the membrane in each well was analyzed in triplicate and repeated three times with similar results. Data are mean ± SEM. *P < 0.05, ** P < 0.01. f Representative image of the transwell assays described in e. Red scale bar, 100 μm. g, h TEL2 overexpression attenuated hypoxia-induced migration and invasion in S26 cells. h Representative image of the transwell assays described in g. Red scale bar, 100 μm