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Fig. 4 | Cancer Communications

Fig. 4

From: Overexpression of amplified in breast cancer 1 (AIB1) gene promotes lung adenocarcinoma aggressiveness in vitro and in vivo by upregulating C-X-C motif chemokine receptor 4

Fig. 4

The associations of AIB1 and C-X-C motif chemokine receptor 4 (CXCR4) expression in lung adenocarcinoma cells. a The five genes, CXCR4, tumor necrosis factor (ligand) superfamily member 10 (TNFSF10), matrix metallopeptidase 11 (MMP11), matrix metallopeptidase 2 (MMP2), and vascular endothelial growth factor A (VEGFA), showed more than a 3.5-fold mRNA differential expression in shAIB1-transfected H1993 cells compared with that in control H1993 cells, as shown by using a human tumor metastasis RT2 profiler PCR array. b Silencing of AIB1 by two shRNAs down-regulated CXCR4 expression in shAIB1 H1993 cells, as detected by Western blotting. c Upper left: treatment of 2 CXCR4-shRNAs in A549-AIB1 cells efficiently decreased the expression levels of CXCR4 as detected by Western blotting. Upper right and down: wound-healing assay showed that the enhanced migrative ability in A549-AIB1 cells was inhibited by silencing CXCR4. d Transwell assay demonstrated that the increased invasive capacity of A549-AIB1 cells was suppressed by CXCR4 silencing. Data are the mean ± SE of three independent experiments. **P < 0.01, *P < 0.05 versus cells transfected with A549-AIB1 by Student’s t test. e Upper left: the level of CXCR4 decreased by silence of AIB1, and then increased after the treatment of CXCR4 as detected by Western blotting. Upper right and down: Wound-healing assay showed that the attenuated migrative ability in H1993-shAIB1 cells was enhanced by the overexpression of CXCR4. f Transwell assay demonstrated that the attenuated invasive capacity of H1993-shAIB1 cells was enhanced by the overexpression of CXCR4. Data are the mean ± SE of three independent experiments. **P < 0.01, *P < 0.05 versus cells transfected with AIB1-shNC by Student’s t test

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