Fig. 5
From: Phenformin alone or combined with gefitinib inhibits bladder cancer via AMPK and EGFR pathways
Evaluation of colony suppression by phenformin combined with gefitinib. a–c Cells were treated for 7 days with phenformin alone, gefitinib alone or both, and then stained with crystal violet to allow colony counting. MB49 cells were treated with 0.125 mmol/L phenformin, 0.125 µmol/L gefitinib, or both; T24 cells, with 0.125 mmol/L phenformin, 1 µmol/L gefitinib, or both; UMUC3 cells, with 0.0125 mmol/L phenformin, 1 µmol/L gefitinib, or both. Wells were photographed using an inverted microscope (magnification, ×10). Control wells contained no drug. d–f Quantification of the experiments conducted in panels (a–c). Wells were scanned at a wavelength of 550 nm. Results are the mean ± SD of five independent experiments. *P < 0.05, #P < 0.01 vs. control (two-tailed t test)