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Fig. 4 | Cancer Communications

Fig. 4

From: Phenformin alone or combined with gefitinib inhibits bladder cancer via AMPK and EGFR pathways

Fig. 4

Evaluation of colony suppression by phenformin on three bladder cancer cell lines. ac Cells were treated for 7 days with phenformin alone, and then stained with crystal violet to allow colony counting. MB49 and T24 cells were treated with 0–0.5 mmol/L phenformin; UMUC3 cells, with 0–0.05 mmol/L. Wells were photographed using an inverted microscope (magnification, ×10). Control wells contained no drug. df Quantification of the experiments conducted in panels (ac). Wells were scanned at a wavelength of 550 nm. Results are the mean ± SD of five independent experiments. *P < 0.05, #P < 0.01 vs. control (two-tailed t test)

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