Fig. 4
From: Phenformin alone or combined with gefitinib inhibits bladder cancer via AMPK and EGFR pathways
Evaluation of colony suppression by phenformin on three bladder cancer cell lines. a–c Cells were treated for 7 days with phenformin alone, and then stained with crystal violet to allow colony counting. MB49 and T24 cells were treated with 0–0.5 mmol/L phenformin; UMUC3 cells, with 0–0.05 mmol/L. Wells were photographed using an inverted microscope (magnification, ×10). Control wells contained no drug. d–f Quantification of the experiments conducted in panels (a–c). Wells were scanned at a wavelength of 550 nm. Results are the mean ± SD of five independent experiments. *P < 0.05, #P < 0.01 vs. control (two-tailed t test)