Table 1 PCR primers and fragments for RAS/BRAF testing (KRAS exons 2, 3, and 4; NRAS exons 2, 3, and 4; and BRAF exon 15)
Exon | Primer | Fragment length (bp) |
|---|---|---|
KRAS | ||
 Exon 2 | F: 5′-TACTGGTGGAGTATTTGATAG-3′ | 248 |
R: 5′-TGGTCCTGCACCAGTAATATG-3′ | ||
 Exon 3 | F: 5′-GCACTGTAATAATCCAGACTGTG-3′ | 222 |
R: 5′-CCCACCTATAATGGTGAATATCTTC-3′ | ||
 Exon 4 | F: 5′-ATGACAAAAGTTGTGGACAGGTTTTGA-3′ | 284 |
R: 5′-ATGATTTTGCAGAAAACAGATCTGTATTTATTTCAG-3′ | ||
NRAS | ||
 Exon 2 | F: 5′-GAACCAAATGGAAGGTCACACT-3′ | 243 |
R: 5′-CCTCACCTCTATGGTGGGATC-3′ | ||
 Exon 3 | F: 5′-TAGCATTGCATTCCCTGTGGTT-3′ | 258 |
R: 5′-CCTGTAGAGGTTAATATCCGCAA-3′ | ||
 Exon 4 | F: 5′-GCCACTGTACCCAGCCTAATCTTG-3′ | 287 |
R: 5′-CACATCTCTACCAGAGTTAATCAACTGATGC-3′ | ||
BRAF | ||
 Exon 15 | F: 5′-CCTAAACTCTTCATAATGCTTGCTC-3′ | 211 |
R: 5′-GTGGAAAAATAGCCTCAATTCTTACC-3′ | ||