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Table 1 PCR primers and fragments for RAS/BRAF testing (KRAS exons 2, 3, and 4; NRAS exons 2, 3, and 4; and BRAF exon 15)

From: Clinicopathologic features and treatment efficacy of Chinese patients with BRAF-mutated metastatic colorectal cancer: a retrospective observational study

Exon Primer Fragment length (bp)
KRAS
 Exon 2 F: 5′-TACTGGTGGAGTATTTGATAG-3′ 248
R: 5′-TGGTCCTGCACCAGTAATATG-3′
 Exon 3 F: 5′-GCACTGTAATAATCCAGACTGTG-3′ 222
R: 5′-CCCACCTATAATGGTGAATATCTTC-3′
 Exon 4 F: 5′-ATGACAAAAGTTGTGGACAGGTTTTGA-3′ 284
R: 5′-ATGATTTTGCAGAAAACAGATCTGTATTTATTTCAG-3′
NRAS
 Exon 2 F: 5′-GAACCAAATGGAAGGTCACACT-3′ 243
R: 5′-CCTCACCTCTATGGTGGGATC-3′
 Exon 3 F: 5′-TAGCATTGCATTCCCTGTGGTT-3′ 258
R: 5′-CCTGTAGAGGTTAATATCCGCAA-3′
 Exon 4 F: 5′-GCCACTGTACCCAGCCTAATCTTG-3′ 287
R: 5′-CACATCTCTACCAGAGTTAATCAACTGATGC-3′
BRAF
 Exon 15 F: 5′-CCTAAACTCTTCATAATGCTTGCTC-3′ 211
R: 5′-GTGGAAAAATAGCCTCAATTCTTACC-3′
  1. RAS rat sarcoma, BRAF v-raf murine sarcoma viral oncogene homolog B1, KRAS Kirsten rat sarcoma viral oncogene homolog, NRAS neuroblastoma RAS viral oncogene homolog