Fig. 4

Effects of TRIM3 overexpression on the cell cycle and apoptosis of HepG2 and Bel-7402 cells. a, b Effect of TRIM3 overexpression on the cell cycle was determined using flow cytometry. TRIM3 overexpression caused G₀/G₁ phase arrest in HepG2 (a) and Bel-7402 (b) cells. c, d Effect of TRIM3 overexpression on apoptosis of HepG2 and Bel-7402 cells was determined with Annexin V-propidium iodide (PI) staining method. Apoptosis rates are not significantly different between HepG2 (c) and Bel-7402 (d) cells infected with LV-TRIM3 and those infected with LV-NC. P values were calculated using Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001, versus cells infected with LV-NC