Fig. 1

The expression of tripartite motif-containing 3 (TRIM3) protein in liver cancer cell lines evaluated with Western blotting. a Compared with the normal liver cell line L02, Bel-7402 and HepG2 cell lines have much lower levels of TRIM3. Relative protein levels of TRIM3 in different cell lines are shown as mean ± standard deviation (SD). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was used as internal reference. b TRIM3 expression in HepG2 and Bel-7402 cells infected with lentiviruses expressing TRIM3 (LV-TRIM3) at multiplicities of infection (MOIs) of 50, 100, 200, and 400 was higher than that in cells infected with negative control vector (LV-NC, MOI of 50) on day 16 after infection. HepG2 and Bel-7402 cells infected with lentiviruses at an MOI of 200 were selected to be used in function assays. c Among the three TRIM3-targeted small interfering RNAs (siTRIM3), siTRIM3 #1 and siTRIM3 #2 showed higher knockdown efficiencies after 48-h transfection. siNC, negative control small interfering RNAs