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Table 1 Primary antibodies and conditions used for immunohistochemical staining

From: CD30 expression in extranodal natural killer/T-cell lymphoma, nasal type among 622 cases of mature T-cell and natural killer-cell lymphoma at a single institution in South China

Primary antibody Manufacturer (Cat. No.) Pretreatment Working dilution
Mouse anti-CD20 monoclonal antibody Zymed, San Diego, CA, USA (18-0155) HP CB (pH 6.0) 1:100
Mouse anti-CD79A monoclonal antibody Novocastra, New Castle, Tyne and Wear, UK (RTU-CD79α-192) HP CB (pH 6.0) Ready to use
Mouse anti-CD3ε monoclonal antibody Novocastra, New Castle, Tyne and Wear, UK (RTU-CD3-PS1) HP CB (pH 6.0) Ready to use
Mouse anti-CD45RO monoclonal antibody Zymed, San Diego, CA, USA (08-1365) HP CB (pH 6.0) Ready to use
Mouse anti-CD5 monoclonal antibody Zymed, San Diego, CA, USA (08-1283) HP CB (pH 6.0) Ready to use
Mouse anti-CD10 monoclonal antibody Novocastra, New Castle, Tyne and Wear, UK (RTU-CD10-270) HP CB (pH 6.0) Ready to use
Mouse anti-CD23 monoclonal antibody Novocastra, New Castle, Tyne and Wear, UK (RTU-CD23-1B12) HP CB (pH 6.0) Ready to use
Mouse anti-CD30 monoclonal antibody Zymed, San Diego, CA, USA (08-0155) HP CB (pH 6.0) Ready to use
Mouse anti-CD56 monoclonal antibody Zymed, San Diego, CA, USA (08-0152) HP CB (pH 6.0) Ready to use
Mouse anti-Bcl-6 monoclonal antibody Zymed, San Diego, CA, USA (08-1426) HP EDTA (pH 9.0) Ready to use
Mouse anti-ALK monoclonal antibody Zeta, Sierra Madre, CA, USA (Z2035) HP EDTA (pH 8.0) Ready to use
Mouse anti-Ki-67 monoclonal antibody Zymed, San Diego, CA, USA (18-0192Z) HP CB (pH 6.0) 1:50
Mouse anti-TdT monoclonal antibody Zymed, San Diego, CA, USA (18-7237) HP EDTA (pH 9.0) 1:50
Mouse anti-TIA-1 monoclonal antibody Zeta, Sierra Madre, CA, USA (Z2183) HP CB (pH 6.0) Ready to use
Mouse anti-Perforin monoclonal antibody Zeta, Sierra Madre, CA, USA (Z2118) HP CB (pH 6.0) Ready to use
Mouse anti-Granzyme B monoclonal antibody Newmarket Scientific, Newmarket, Suffolk, UK (MS-1157-s) HP CB (pH 6.0) Ready to use
Mouse anti-PD-1 monoclonal antibody Zymed, San Diego, CA, USA (0381) HP CB (pH 6.0) 1:100
Goat anti-CXCL13 polyclonal antibody Zymed, San Diego, CA, USA (0043) HP CB (pH 6.0) 1:100
Mouse anti-CD4 monoclonal antibody Zymed, San Diego, CA, USA (0032) HP CB (pH 6.0) 1:200
Rabbit anti-CD8 monoclonal antibody Zymed, San Diego, CA, USA (0043) HP CB (pH 6.0) 1:100
  1. HP high pressure (boiled with buffer in a stainless steel high-pressure cooker for 10 min, with the pressure setting at approximately 103 kPa), CB 0.01 mol/L citrate buffer, EDTA 0.01 mol/L ethylenediaminetetraacetic acid, Bcl-6 B-cell lymphoma-6, ALK anaplastic large cell lymphoma kinase, TdT terminal deoxynucleotidyl transferase, TIA-1 T-cell-restricted intracellular antigen-1, PD-1 programmed cell death 1, CXCL13 C-X-C motif chemokine ligand 13