Advertisement
Table 1 Primary antibodies and conditions used for immunohistochemical staining
| Primary antibody | Manufacturer (Cat. No.) | Pretreatment | Working dilution |
|---|---|---|---|
| Mouse anti-CD20 monoclonal antibody | Zymed, San Diego, CA, USA (18-0155) | HP CB (pH 6.0) | 1:100 |
| Mouse anti-CD79A monoclonal antibody | Novocastra, New Castle, Tyne and Wear, UK (RTU-CD79α-192) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD3ε monoclonal antibody | Novocastra, New Castle, Tyne and Wear, UK (RTU-CD3-PS1) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD45RO monoclonal antibody | Zymed, San Diego, CA, USA (08-1365) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD5 monoclonal antibody | Zymed, San Diego, CA, USA (08-1283) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD10 monoclonal antibody | Novocastra, New Castle, Tyne and Wear, UK (RTU-CD10-270) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD23 monoclonal antibody | Novocastra, New Castle, Tyne and Wear, UK (RTU-CD23-1B12) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD30 monoclonal antibody | Zymed, San Diego, CA, USA (08-0155) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-CD56 monoclonal antibody | Zymed, San Diego, CA, USA (08-0152) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-Bcl-6 monoclonal antibody | Zymed, San Diego, CA, USA (08-1426) | HP EDTA (pH 9.0) | Ready to use |
| Mouse anti-ALK monoclonal antibody | Zeta, Sierra Madre, CA, USA (Z2035) | HP EDTA (pH 8.0) | Ready to use |
| Mouse anti-Ki-67 monoclonal antibody | Zymed, San Diego, CA, USA (18-0192Z) | HP CB (pH 6.0) | 1:50 |
| Mouse anti-TdT monoclonal antibody | Zymed, San Diego, CA, USA (18-7237) | HP EDTA (pH 9.0) | 1:50 |
| Mouse anti-TIA-1 monoclonal antibody | Zeta, Sierra Madre, CA, USA (Z2183) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-Perforin monoclonal antibody | Zeta, Sierra Madre, CA, USA (Z2118) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-Granzyme B monoclonal antibody | Newmarket Scientific, Newmarket, Suffolk, UK (MS-1157-s) | HP CB (pH 6.0) | Ready to use |
| Mouse anti-PD-1 monoclonal antibody | Zymed, San Diego, CA, USA (0381) | HP CB (pH 6.0) | 1:100 |
| Goat anti-CXCL13 polyclonal antibody | Zymed, San Diego, CA, USA (0043) | HP CB (pH 6.0) | 1:100 |
| Mouse anti-CD4 monoclonal antibody | Zymed, San Diego, CA, USA (0032) | HP CB (pH 6.0) | 1:200 |
| Rabbit anti-CD8 monoclonal antibody | Zymed, San Diego, CA, USA (0043) | HP CB (pH 6.0) | 1:100 |
