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TableĀ 1 Results of RQ-PCR for AML1-ETO fusion transcript detection and 3D-FISH for chromosomal organization detection in a 35-year-old male patient with t(8;21)(q22;q22)-positive AML-M2

From: Significance of spatial organization of chromosomes in the progression of acute myeloid leukemia

Disease course AML1-ETO levela detected with RQ-PCR (%) Percentage of cells detected with 3D-FISH
Labeling chromosomes 8 and 21 Labeling chromosomes 8 and 18
Normal cells (%) Proximal cells (%) Malignant cells (%) Normal cells (%) Proximal cells (%) Malignant cells
CR2 0.1 54.2 41.6 4.2 NA NA NA
Post-HSCT 0.0 55.2 29.9 14.9 61.4 30.0 8.6% (8.6% for 3g2r)
Follow-up 1 0.0 42.9 34.9 22.2 46.5 39.4 14.1% (11.3% for 3g2r)
Follow-up 2 0.0 36.1 34.4 29.5 47.9 39.8 12.3% (10.8% for 3g2r)
Relapse 28.6 32.8 29.5 37.7 45.4 47.0 7.6% (6.1% for 3g2r)
  1. RQ-PCR, real-time fluorescent quantitative polymerase chain reaction; AML1-ETO, acute myeloid leukemia factor 1-eight-twenty-one; 3D-FISH, three-dimensional fluorescence in situ hybridization; AML-M2, acute myeloid leukemia with maturation; CR2, second complete remission; HSCT, hematopoietic stem cell transplantation; 3g2r, three green signals and two red signals, indicating the break of chromosome 8; NA, not applicable (only chromosomes 8 and 21 were labeled and analyzed for the CR2 sample)
  2. aNormalized level of AML1-ETO transcripts was calculated by dividing the total AML1-ETO copy number by the total ABL copy number