Table 1 Results of RQ-PCR for AML1-ETO fusion transcript detection and 3D-FISH for chromosomal organization detection in a 35-year-old male patient with t(8;21)(q22;q22)-positive AML-M2
From: Significance of spatial organization of chromosomes in the progression of acute myeloid leukemia
Disease course | AML1-ETO levela detected with RQ-PCR (%) | Percentage of cells detected with 3D-FISH | |||||
|---|---|---|---|---|---|---|---|
Labeling chromosomes 8 and 21 | Labeling chromosomes 8 and 18 | ||||||
Normal cells (%) | Proximal cells (%) | Malignant cells (%) | Normal cells (%) | Proximal cells (%) | Malignant cells | ||
CR2 | 0.1 | 54.2 | 41.6 | 4.2 | NA | NA | NA |
Post-HSCT | 0.0 | 55.2 | 29.9 | 14.9 | 61.4 | 30.0 | 8.6% (8.6% for 3g2r) |
Follow-up 1 | 0.0 | 42.9 | 34.9 | 22.2 | 46.5 | 39.4 | 14.1% (11.3% for 3g2r) |
Follow-up 2 | 0.0 | 36.1 | 34.4 | 29.5 | 47.9 | 39.8 | 12.3% (10.8% for 3g2r) |
Relapse | 28.6 | 32.8 | 29.5 | 37.7 | 45.4 | 47.0 | 7.6% (6.1% for 3g2r) |