Fig. 1

Real-time fluorescent quantitative polymerase chain reaction analysis of the bone marrow sample extracted when the patient with t(8;21)(q22;q22)-positive acute myeloid leukemia with maturation (AML-M2) relapsed after induction chemotherapy and hematopoietic stem cell transplantation. a Amplification plots of AML1-ETO (acute myeloid leukemia factor 1-eight-twenty-one), positive control, negative control, and three standard concentrations (10³, 10⁴, and 10⁶ copies) of the AML1-ETO gene. b Amplification plots of ABL (Abelson) and three standard concentrations (10³, 10⁴, and 10⁶ copies) of the ABL gene. c Standard curve of the AML1-ETO gene copy number. Y = −3.25lg(X) + 38.19, R ² = 0.999,67. The negative control gene product was undetectable. d Standard curve of the ABL gene copy number. Y = −3.50lg(X) + 42.96, R ² = 0.999,98