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Table 3 ASB3 gene mutations in colorectal cancer tissues and cell lines

From: The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells

Mutation genotype

Mutation type

Exon

Codon change

Amino acid change

Case ID/Cell line

Colorectal cancer tissues

 

 CDS 21C→Ga

Nonsense

Exon 2

TAC→TAG b

Y7stop

C56

 chr2:53810098 G→C

Splice-site

Exon 3/Intron

C53

 CDS 404G→R

Missense

Exon 4

GGA→GAA

G135E

S78, S64

 CDS 1084C→Y

Missense

Exon 8

CGC→TGC

R362C

S35

 CDS 1016A→W

Missense

Exon 8

AAA→ATA

K339I

S12, S61, S64

Colorectal cancer cell lines

 

 CDS 471A→T

Missense

Exon 5

GAA→GAT

E157D

HCT116

 CDS 471A→W

Missense

Exon 5

GAA→GAT

E157D

HT-29

 CDS 24G→R

Synonymous

Exon 2

GCG→GCA

Non

DLD-1

 CDS 25G→K

Missense

Exon 2

GAC→TAC

D9Y

DLD-1

 CDS 154T→Y

Missense

Exon 2

TAT→CAT

Y52H

DLD-1

 CDS 922G→R

Missensee

Exon 7

GCC→ACC

A308T

DLD-1

  1. ASB3, ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3
  2. The nucleotides in bold indicate the one mutated
  3. a CDS coding domain sequence; A, T, C, G, four types of nucleotides; R = A/G; Y = C/T; W = A/T; K = G/T
  4. bamino acid codon