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Table 2 The sequences of primers used in this study

From: The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells

Target Direction Sequences
For qPCR
ASB3 Forward 5′-CATACTTATTTCATCGGGTGC-3′
Reverse 5′-GGTAACTGCCAACTGTCCTC-3′
GAPDH Forward 5′-CTCCTCCTGTTCGACAGTCAGC-3′
Reverse 5′-CCCAATACGACCAAATCCGTT-3′
For vector construction (italics indicates restriction enzyme recognition sequence)
WT ASB3 Forward 5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′
Reverse 5′-GGCGCGGCCGCTTATCCATCTTGAATAGCTG-3′
ΔSOCS Forward 5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′
Reverse 5′-GGGATCGATTTACCTTTCAACAGCTGGTG-3′
For point mutation generation (capital letter indicates mutated nucleotide)
G135E Forward 5′- ggctgttgcttcaacacgAagcaaatgttaatggatc-3′
Reverse 5′-gatccattaacatttgctTcgtgttgaagcaacagcc-3′
K339I Forward 5′-gtgaacattcttttgaTatatggagcccagata-3′
Reverse 5′-tatctgggctccatatAtcaaaagaatgttcac-3′
R362C Forward 5′-cgagaagttttcgatatttTgctactttttgaggaaagg-3′
Reverse 5′-cctttcctcaaaaagtagcAaaatatcgaaaacttctcg-3′
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Cancer Communications

ISSN: 2523-3548

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