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Table 2 The sequences of primers used in this study
| Target | Direction | Sequences |
|---|---|---|
| For qPCR | ||
| ASB3 | Forward | 5′-CATACTTATTTCATCGGGTGC-3′ |
| Reverse | 5′-GGTAACTGCCAACTGTCCTC-3′ | |
| GAPDH | Forward | 5′-CTCCTCCTGTTCGACAGTCAGC-3′ |
| Reverse | 5′-CCCAATACGACCAAATCCGTT-3′ | |
| For vector construction (italics indicates restriction enzyme recognition sequence) | ||
| WT ASB3 | Forward | 5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′ |
| Reverse | 5′-GGCGCGGCCGCTTATCCATCTTGAATAGCTG-3′ | |
| ΔSOCS | Forward | 5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′ |
| Reverse | 5′-GGGATCGATTTACCTTTCAACAGCTGGTG-3′ | |
| For point mutation generation (capital letter indicates mutated nucleotide) | ||
| G135E | Forward | 5′- ggctgttgcttcaacacgAagcaaatgttaatggatc-3′ |
| Reverse | 5′-gatccattaacatttgctTcgtgttgaagcaacagcc-3′ | |
| K339I | Forward | 5′-gtgaacattcttttgaTatatggagcccagata-3′ |
| Reverse | 5′-tatctgggctccatatAtcaaaagaatgttcac-3′ | |
| R362C | Forward | 5′-cgagaagttttcgatatttTgctactttttgaggaaagg-3′ |
| Reverse | 5′-cctttcctcaaaaagtagcAaaatatcgaaaacttctcg-3′ | |
