For qPCR
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ASB3
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Forward
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5′-CATACTTATTTCATCGGGTGC-3′
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Reverse
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5′-GGTAACTGCCAACTGTCCTC-3′
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GAPDH
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Forward
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5′-CTCCTCCTGTTCGACAGTCAGC-3′
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Reverse
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5′-CCCAATACGACCAAATCCGTT-3′
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For vector construction (italics indicates restriction enzyme recognition sequence)
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WT ASB3
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Forward
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5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′
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Reverse
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5′-GGCGCGGCCGCTTATCCATCTTGAATAGCTG-3′
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ΔSOCS
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Forward
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5′-GCGCTCGAGATGGATTTTACAGAGGCT-3′
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Reverse
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5′-GGGATCGATTTACCTTTCAACAGCTGGTG-3′
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For point mutation generation (capital letter indicates mutated nucleotide)
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G135E
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Forward
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5′- ggctgttgcttcaacacgAagcaaatgttaatggatc-3′
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Reverse
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5′-gatccattaacatttgctTcgtgttgaagcaacagcc-3′
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K339I
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Forward
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5′-gtgaacattcttttgaTatatggagcccagata-3′
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Reverse
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5′-tatctgggctccatatAtcaaaagaatgttcac-3′
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R362C
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Forward
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5′-cgagaagttttcgatatttTgctactttttgaggaaagg-3′
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Reverse
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5′-cctttcctcaaaaagtagcAaaatatcgaaaacttctcg-3′
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