Fig. 3

The radioresistant non-small cell lung cancer cells showed enhanced ability of invasion and migration. a, b Transwell matrigel invasion and migration assays with A549, A549R, H1299, and H1299R cells. The cells were stained with crystal violet, counted under a light microscope, and photographed at 200× magnification. The cell number was normalized to A549 or H1299 cells, respectively, and are presented as mean ± standard deviation (SD). **P < 0.01 (Student’s t test). c, d Wound healing assay with A549, A549R, H1299, and H1299R cells. Representative pictures are shown, and the width of the wounds was measured at 0 and 24 h after the scratch. The migration rate was normalized to A549 or H1299 cells, respectively, and is presented as mean ± SD. **P < 0.01 (Student’s t test)