Fig. 1
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40880-015-0040-8/MediaObjects/40880_2015_40_Fig1_HTML.gif)
Preprocessing steps of our analysis. Short reads as provided in Fastq files are aligned with Bowtie 2, resulting in Sam files. Application of our method Depth of Sequencing Iterative Reduction Estimator (DESIRE) extracts a defined subsample of size f, resulting in updated Sam files. Finally, feature Counts, a function from the R subread package, is applied to obtain the count vector for one sample.