Fig. 5
FOXM1–Survivin axis was up-regulated in TMZ-insensitive glioma cells. a RT-qPCR (mRNA level, upper part) and Western blotting (protein level, lower part) measuring the expression of FOXM1 and Survivin in TMZ-insensitive U251 and U87 cells induced by 200 μmol/L and 500 μmol/L TMZ (In200-U251/U87 and In500-U251/U87). b Immunofluorescent staining of FOXM1 in normal (Control) and In500-U251/U87 cells (scale bar, 100 μm). c RT-qPCR detecting mRNA levels of FOXM1 and Survivin in In500-U251/U87 cells after cultivating in medium with 500 μmol/L TMZ or equivalent DMSO for 7 days. d Immunofluorescent staining of FOXM1 protein in In500-U251/U87 cells after cultivating in medium with 500 μmol/L TMZ or equivalent DMSO for 7 days (scale bar, 100 μm). e Cell viability rates were calculated after In500-U251/U87 cells were treated with 10 or 20 nmol/L bortezomib, 800 μmol/L TMZ, bortezomib + TMZ, or drug vehicle (DMSO, control) for 48 h. *P < 0.05; **P < 0.01. RT-qPCR reverse transcription-quantitative polymerase chain reaction, TMZ temozolomide, DAPI 4′,6-diamidino-2-phenylindole, DMSO dimethyl sulfoxide, Bor bortezomib, ns no significance