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Fig. 3 | Cancer Communications

Fig. 3

From: Bortezomib inhibits growth and sensitizes glioma to temozolomide (TMZ) via down-regulating the FOXM1–Survivin axis

Fig. 3

Bortezomib down-regulated the FOXM1–Survivin axis in glioma cells. a Left part, measuring FOXM1 in U251 and U87 after treatment with 0 (Control, DMSO), 10, or 20 nmol/L bortezomib for 48 h at the mRNA level (RT-qPCR, left upper part) and protein level (Western blotting, left lower part). Right part, immunofluorescent staining of FOXM1 in U251 and U87 cells after bortezomib treatment for 48 h (scale bar, 100 μm). b Same number of FOXM1-overexpressed, FOXM1 siRNA-transfected, and EV-transfected cells were treated with bortezomib for 48 h. Cell viability was measured by MTT assay. c FOXM1 and Survivin mRNA levels in 6 glioma cell lines were detected by RT-qPCR. The correlation between FOXM1 and Survivin mRNA levels was analyzed via GraphPad Prism 7.0 using Pearson R test. d FOXM1 and Survivin mRNA (RT-qPCR, left part) and protein expression (Western blotting, right part) in FOXM1-overexpressed, FOXM1 siRNA-transfected, and EV-transfected cells. e Left part, Survivin mRNA (RT-qPCR, left upper part) and protein expression (Western blotting, left lower part) in U251 and U87 cells after treatment with bortezomib for 48 h. Right part, immunofluorescent staining of Survivin in U251 and U87 cells after treatment with bortezomib for 48 h (scale bar, 100 μm). *P < 0.05; **P < 0.01. RT-qPCR reverse transcription-quantitative polymerase chain reaction, DMSO dimethyl sulfoxide, GAPDH glyceraldehyde-3-phosphate dehydrogenase, DAPI 4′,6-diamidino-2-phenylindole, siRNA short interfering RNA, Bor bortezomib, EV empty vector, OE overexpression, MTT 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide

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