Polymorphisms of MTHFR and TYMS predict capecitabine-induced hand-foot syndrome in patients with metastatic breast cancer

Shaoyan Lin; Jian Yue; Xiuwen Guan; Peng Yuan; Jiayu Wang; Yang Luo; Ying Fan; Ruigang Cai; Qiao Li; Shanshan Chen; Pin Zhang; Qing Li; Fei Ma; Binghe Xu

doi:10.1186/s40880-019-0399-z

Table 1 The polymerase chain reaction (PCR) primers and homogeneous Mass EXTEND (hME) primers of 22 single nucleotide polymorphisms (SNPs) in three candidate genes

From: Polymorphisms of MTHFR and TYMS predict capecitabine-induced hand-foot syndrome in patients with metastatic breast cancer

Gene	Localization	SNP	PCR primer (5′ → 3′)	hME primer (5′ → 3′)
TYMS	18p11.32	2790	First: ACGTTGGATGGGATGCCGAGGTAAAAGTTC	GATTTTTGACCTAGTTCCTT
		2790	Second: ACGTTGGATGAACTGATAGGTCACGGACAG
		2,853,741	First: ACGTTGGATGGGAAACAGATCTCAAACAGC	GGTACCACGTTTTCCTGCGGTCTTGTC
		2,853,741	Second: ACGTTGGATGAGCACAGTTCCCACGTTTTC
		2,606,241	First: ACGTTGGATGCCACAGCTGAGAGTCTTAGG	GGGCGCAGTCCTTCCC
		2,606,241	Second: ACGTTGGATGACCAGACGGTTCCCAAAGG
		3,786,362	First: ACGTTGGATGTTGGACAGCCTGGGATTCTC	GCCCAAGTCCCCTTC
		3,786,362	Second: ACGTTGGATGCAAAATGCCTCCACTGGAAG
		1,004,474	First: ACGTTGGATGTAAAACTGTGACTCTCCCCC	GACCTCAGATGGTGATGTTCGTCTA
		1,004,474	Second: ACGTTGGATGGGGAAAGGCTGACATACATC
		9,947,507	First: ACGTTGGATGAATTCTTCTGCCTCAGCCTC	GCCCCCGTCTCTACTAAAA
		9,947,507	Second: ACGTTGGATGTGGACAACATGGTGAAACCC
		699,517	First: ACGTTGGATGCCACTGAAGAACCCTAAAAG	GGAGAAAGACTGACAATATCCTTC
		699,517	Second: ACGTTGGATGACTTTTACCTCGGCATCCAG
		9,967,368	First: ACGTTGGATGTGGGTGACAGAGCCGTATG	AACCCAGATATTCCTTTCTATT
		9,967,368	Second: ACGTTGGATGGAATCCATGGTCTCCACAAC
		15,872	First: ACGTTGGATGACAGAACTACACTACCAAGG	CCCCCCTCTCATGGTCACTGTTCC
		15,872	Second: ACGTTGGATGGAAAGTCCTCTCATGGTCAC
MTHFR	1p36.3	1,801,133	First: ACGTTGGATGCTTGAAGGAGAAGGTGTCTG	AACGCGTGATGATGAAATCG
		1,801,133	Second: ACGTTGGATGTGCATGCCTTCACAAAGCGG
		1,801,131	First: ACGTTGGATGTCTCCCGAGAGGTAAAGAAC	CATGAGCTGACCAGTGAAG
		1,801,131	Second: ACGTTGGATGAGGAGCTGCTGAAGATGTGG
		3,737,964	First: ACGTTGGATGTGATGGCTGTAGATCCTCAC	GCAGCCCTCAAAAAAAACCTTTC
		3,737,964	Second: ACGTTGGATGTCAAATAGGAACCAGCCCTC
		4,846,049	First: ACGTTGGATGAACTAAGCCCTCGAACCAAG	TGCACGGGCTCCAAG
		4,846,049	Second: ACGTTGGATGTGTTTTGCCTGTACTGCACG
		2,274,976	First: ACGTTGGATGTATGTGTGTGTAGGACGAGG	CATACAGCTTTCCCCAC
		2,274,976	Second: ACGTTGGATGATGTACTGGATGATGGTGCG
		3,753,582	First: ACGTTGGATGACGCAGTGGGCGCCAGGGA	CTCATTTTAAACCTGCCTCCCCGGCGA
		3,753,582	Second: ACGTTGGATGTGCCTTTTAAACCTGCCTCC
		4,846,048	First: ACGTTGGATGTTTGGTTTGGTGGTGGCTTC	GGAATCAGTTAGTTCTGACACCAACAA
		4,846,048	Second: ACGTTGGATGTCCAGACCAGAAGCAGTTAG
RRM1	11p15.5	725,519	First: ACGTTGGATGCACTTTAACTCTAGAAGATTG	TGGTGAAGAAATATGTAATGCCTCA
		725,519	Second: ACGTTGGATGGCCTAGCATATAAAGTGCTC
		720,106	First: ACGTTGGATGGCAGTAATAAGAGCAGTTATC	AAACATTTATAACAAACTTAACATAC
		720,106	Second: ACGTTGGATGAAGGGTCAAATGAGTACCTC
		1,042,858	First: ACGTTGGATGAGGGTTTGAAGACTGGGATG	GAACTGGATTGGATTAGC
		1,042,858	Second: ACGTTGGATGGCTTCTCCTTATTTAGAGTG
		1,042,927	First: ACGTTGGATGCCACCAGTCAAAGCAGTAAA	GGTAAGTAAGGTTTCATCACCC
		1,042,927	Second: ACGTTGGATGCAGGGAGTGGTTAAGTAAGG
		11,030,918	First: ACGTTGGATGTCCTGACGCAAATCAGAGCC	GGCTTACCCTGCCCTGCTTAAAAT
		11,030,918	Second: ACGTTGGATGCCACTGAAGAACCCTAAAAG
		1,980,412	First: ACGTTGGATGGGTCTTCAGAACTATGAGAG	GGGCCAAAGGTATTTAAGTTTCCTATG
		1,980,412	Second: ACGTTGGATGCCAGAGGACAAAGGTATTTA

TYMS thymidylate synthase, MTHFR methylene tetrahydrofolate reductase, RRM1 ribonucleotide reductase M1

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