Fig. 1

The homogeneous Mass EXTEND (hME) reaction. A deoxyguanosine triphosphate (dGTP) was used along with terminators dideoxyadenosine 5′-triphosphate (ddATP), dideoxycytidine 5′-triphosphate (ddCTP), and dideoxythymidine 5′-triphosphate (ddTTP). For the T allele (T), ddATP was incorporated, extending the primer to a 24-mer. For the C allele (C), dGTP was incorporated prior to the termination of the extension by incorporation of a ddATP, yielding a 25-mer. U primer marked unextended primer