Fig. 3
From: Regulation of CD137 expression through K-Ras signaling in pancreatic cancer cells
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40880-019-0386-4/MediaObjects/40880_2019_386_Fig3_HTML.png)
Role of the NF-κB pathway in mediating K-Ras-induced CD137 expression. a, b Panc-1 and SW1990 pancreatic cancer cells were transfected with 100 nmol/L control siRNA or siRNA against p65 (RELA) for 48 h. CD137 mRNA was measured by real-time PCR. c T-Rex/K-Ras cells were first transfected with 100 nmol/L control siRNA or siRNA against p65 for 24 h, then incubated with doxycycline for 48 h. CD137 mRNA was measured by real-time PCR. d HCT116 cells were transfected with 100 nmol/L siRNA against p65 or control RNA (siCTL) for 48 h, and CD137 mRNA was measured by real-time PCR. Data are presented as mean ± SEM of three repeated experiments. Statistical analysis: two-tailed unpaired t-test for a–d. **P < 0.01; ***P < 0.001. CD137 tumor necrosis factor receptor superfamily member 9, Doxy doxycycline, siCTL control siRNA, sip65 siRNA against p65, PCR polymerase chain reaction