Fig. 4

Chr9p24 localized genes display region-wide mRNA expression correlation with PD-L1 in LUSC. a Illustration of genes located at Chr9p24 from UCSC browser. 9pTel illustrates the telomere end for the displayed region and 9Cen illustrates the direction for the localization of the Chr9 centromere region. b Diagram illustrating expression correlation of the genes illustrated in a with PD-L1 and PD-L2 in LUAD, LUSC, and Lung_NSC. Red color indicates expression correlation with PD-L1, the blue color indicates expression correlation with PD-L2, and purple color indicates expression correlation with both PD-L1 and PD-L2. c, d Heat map analyses of mRNA expression Z-scores for PD-L1 expression correlated genes located at Chr9p24 for individual patients in LUSC c and LUAD d. The heat maps are sorted relative to PD-L1 mRNA expression and with the vertical gene order reflecting the position at Chr9p24. Spearman and Pearson correlation coefficients r and their corresponding P values between mRNA expression for genes of interest and PD-L1 protein expression in LUAD (n = 365) and LUSC (n = 328) are shown on the right. Correlations assigned significant are shown in red. The criteria for significant expression correlation were Pearson correlation coefficient r ≥ 0.3 or ≤ − 0.3, Spearman correlation coefficient r ≥ 0.4 or ≤ − 0.4, and P values < 0.05. Pe Pearson, r correlation coefficient, Sp Spearman