Fig. 1

PD-L1 and PD-L2 expression correlation analyses in 114 NSCLC cell lines from the CCLE dataset (Lung_NSC). a Overview of key factors interacting with PD-L1 and PD-L2 in NSCLC. PD-L1 and PD-L2 are ligands of PD-1 and the interaction of PD-L1 or PD-L2 with PD-1 results in T cell suppression. Moreover, PD-L1 can interact with CD80 on activated T cells and inhibit T cell activity. PD-L2 has a second receptor, RGMB, and this interaction can activate T cells. b Expression correlation analysis of PD-L1 and PD-L2 in the CCLE dataset (Lung_NSC, n = 114) using Broad portal analysis of RNA sequencing-based gene expression data. Spearman and Pearson correlation coefficients r and the corresponding P value are shown. c Gene expression correlation between PD-1, CD80, RGMB, PD-L1, and PD-L2 in CCLE dataset (Lung_NSC, n = 114) Spearman and Pearson correlation coefficients r and the corresponding P values are shown. The horizontal lines show the pairwise comparison of genes and line thickness is proportional to the degree of expression correlation. Dashed lines indicate negative expression correlation. Significant expression correlation is indicated in red. d Number of genes expression correlated with PD-L1, PD-L2, and both in CCLE dataset (Lung_NSC, n = 114). For all panels, the criteria for significant expression correlation were Pearson correlation coefficient r ≥ 0.3 or ≤ − 0.3, Spearman correlation coefficient r ≥ 0.4 or ≤ − 0.4, and P values < 0.05. The analysis was performed using the GenomicScape portal. BMP bone morphogenic protein, BMPR I BMP receptor type 1, BMPR II BMP receptor type 2, C2-type constant 2-type, CD80 cluster of differentiation 80, IFN interferon, Ig-like Immunoglobulin-like, PD-1 programmed cell death protein-1, PD-L1 programmed cell death ligand-1, PD-L2 programmed cell death ligand-2, RGMB repulsive guidance molecule B, SHP-1/2 Src homology 2 domain-containing protein tyrosine phosphatase 1 and 2, Pe Pearson, r correlation coefficient, Sp Spearman, V-type variable-type