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Fig. 3 | Cancer Communications

Fig. 3

From: Metabolic reprogramming and redox adaptation in sorafenib-resistant leukemia cells: detected by untargeted metabolomics and stable isotope tracing analysis

Fig. 3

Illustration of the decreased glucose flux into the pentose phosphate pathway (PPP) in BaF3/ITD-R cells. a Schematic of [1,2-13C2]-glucose metabolism. The labeled glucose is converted to M + 1 pyruvate through the PPP and M + 2 pyruvate through glycolysis, respectively. b Quantification of mass isotopomer distribution of pyruvate in BaF3/ITD and BaF3/ITD-R cells cultured in [1,2-13C2]-glucose for 24 h. c Glucose uptake and lactate secretion in BaF3/ITD and BaF3/ITD-R cells as described in “Materials and methods” section . d The glucose flux through glycolysis and the PPP in BaF3/ITD and BaF3/ITD-R cells was calculated as described in “Materials and methods” section. e Western blot analysis of TKT and G6PD in BaF3, BaF3/ITD-R, MV4-11 and MV4-11-R cells. Bars, mean ± SEM (Standard Error of the Mean). *P < 0.05, **P < 0.01, ***P < 0.001. PPP pentose phosphate pathway; TKT transketolase; G6PD glucose-6-phosphate dehydrogenase

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