Table 1 Overview of genome-wide 5hmC quantification methods
Technique | Advantages | Disadvantages |
|---|---|---|
Whole-genome TAB-Seq [70] (direct measurement of 5hmC) | Whole-genome Single nucleotide resolution Absolute methylation value | High sequencing depth required Input > 100 ng DNA |
RRBS TAB-Seq [91] (direct measurement of 5hmC) | Single nucleotide resolution Absolute methylation value | Targets CpG islands Input > 500 ng DNA |
RRBS oxBS-Seq [52] (inference of 5hmC) | ~ 60 million PE reads/sample | Input > 500 ng DNA |
Nano-hmC-Seal [53] (direct measurement of 5hmC) | Input 5 ng DNA Excellent genomic coverage High specificity for 5hmC ~ 30 million PE reads/sample | Lower resolution |