Fig. 3

Overexpression of TES suppresses migration and invasion of GC cells both in vitro and in vivo. a Representative pictures show that the Ad-TES- and Ad-Control-transfected SGC7901 and MKN45 cells invaded through the matrigel. Magnification: ×100. b Invaded, Ad-TES-transfected SGC7901 and MKN45 cells were quantified. Ad-Control-transfected SGC7901 and MKN45 cells were used as controls. The results are expressed as mean ± SD of at least three independent experiments. Student’s t-test, *P < 0.05. c Representative pictures show that the Ad-TES- and Ad-Control-transfected SGC7901 and MKN45 cells migrated through the uncoated transwell membrane. Magnification: ×100. d Migrated Ad-TES transfected SGC7901 and MKN45 cells were quantified. Ad-Control-transfected SGC7901 and MKN45 cells were used as controls. The results are expressed as mean ± SD of at least three independent experiments. Student’s t-test, *P < 0.05. e Representative lungs derived from nude mice inoculated with Ad-TES- or Ad-Control-transfected MKN45 cells are shown. The formation of metastatic nodules at the surface of lungs could be significantly suppressed by TES overexpression. f Metastatic nodules in the lung were quantified 6 weeks after tail vein injection of Ad-TES- and Ad-Control-transfected cells (8 mice per group). The nodules were counted in 10 randomly selected high-power fields under a microscope. The results are expressed as mean ± SD. g Representative hematoxylin and eosin staining pictures of the lung sections from mice inoculated with Ad-TES- and Ad-Control-transfected MKN45 cells. Magnification: ×40