Fig. 5

DUXAP8 negatively regulates expression of KLF2 by rescue experiments. a, b The levels of CDKN1A and KLF2 expression were determined by qPCR in 20 pairs of PC tissues. c, d The levels of CDKN1A and KLF2 mRNA expression were determined by qPCR when BxPC-3 cells were transfected with si-NC, si-CDKN1A or si-KLF2. e The CDKN1A and KLF2 protein levels were determined by Western blot in CDKN1A or KLF2 knockdown BxPC-3 cells. f MTT assays were performed to determine the cell viability of BxPC-3 cells after the transfection of siRNA against CDKN1A or KLF2. g, h MTT and colony formation assays were used to determine the cell proliferation ability for BxPC-3 cells transfected with si-NC and si-DUXAP8 and co-transfected with si-DUXAP8, si-CDKN1A or si-DUXAP8, si-KLF2. Representative images and data based on three independent experiments. Bars: s.d, *P < 0.05, **P < 0.01