Table 1 Antibodies and fluorescent dye used for immunofluorescence
Antibody | Species | Manufacturer | Catalog number | Dilution |
|---|---|---|---|---|
CD31 | Rat monoclonal | BD Pharmingen, Franklin Lakes, NJ, USA | 550275 | 1:50 |
Laminin | Rabbit polyclonal | DAKO, Glostrup, Denmark | Z0097 | 1:200 |
Collagen I | Rabbit polyclonal | Chemicon, Billerica, MA, USA | AB765P | 1:100 |
SMA | Mouse monoclonal | DAKO, Glostrup, Denmark | M0851 | 1:200 |
panCK-FITC | Mouse monoclonal | DAKO, Glostrup, Denmark | F0859 | 1:100 |
TOTO-3 | Invitrogen, Carlsbad, CA, USA | T3604 | 1:500 |