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Fig. 5 | Cancer Communications

Fig. 5

From: DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways

Fig. 5

Downregulation of DDIT4 activates the mitogen-activated protein kinase (MAPK) and p53 pathways in GC cells. a The total and phosphorylated levels of AKT, mTOR and 4EBP1 in DDIT4-silenced and DDIT4-overexpressing cells were examined by western blot. b The total and phosphorylated levels of MEK1, P42/44-MAPK, BCL-1, BAD, p53, and p21Cip1 in DDIT4-silenced and DDIT4-overexpressing cells were examined by western blot. c, d SGC7901 and BGC823 cells were infected shDDIT4 lentivirus and then were treated with inhibitors specific to p53 (A15201) or MAPK (PD98059). c The protein expression levels of phosphorylated and total ERK, BCL-2, p53 and p21Cip1 were analyzed by western blot. d Cell proliferation was analyzed by CCK8 assay

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