Fig. 3

Effect of DDIT4 silencing and overexpression on GC cell proliferation, apoptosis and cell cycle progression in vitro. a–c SGC7901 cell proliferation rate in response to 0, 10, and 20 µg/mL 5-FU was determined by high-content proliferation assays at various time points. d Representative images of SGC7901 cell colony formation after treatment with 0, 10, or 20 µg/mL 5-FU. The number of colonies was calculated, and the results are depicted in the bar chart. e–g BGC823 cell proliferation rate. h BGC823 cell colony formation assay. i–k GES cell proliferation rate. l GES cell colony formation assay. m Apoptosis in SGC7901, BGC823, and GES cells in response to 0, 10, or 20 µg/mL 5-FU as determined by flow cytometry. n Cell cycle distribution of SGC7901, BGC823, and GES cells treated with 0, 10, or 20 µg/mL 5-FU. ***P < 0.001, **P < 0.01, *P < 0.05