Fig. 3

Aldolase A positively regulated cyclin D1 expression at the transcriptional level in H520 cells. a Immunohistochemistry assays of cyclin D1 in subcutaneous tumor xenografts of H520 were performed. Cyclin D1 expression was inhibited in the shAL group compared with the shNC group. b The mRNA levels of cyclin D1 in the indicated cells were determined by real-time polymerase chain reaction with β-actin as an internal control. The mRNA level of cyclin D1 was lower in the shAL than shNC group. Data are shown as mean ± SD. *P < 0.05 versus shNC. c Degradation rates of cyclin D1 mRNA after transcription inhibition with actinomycin D (0.5 μg/mL) for indicated periods of time. The mRNA half-life of cyclin D1 showed no significant difference between shAL and shNC cells. Data are shown as mean ± SD. d Dimethyl sulfoxide (DMSO) treatment served as control of actinomycin D treatment. The mRNA levels of cyclin D1 fluctuated in medium supplemented with DMSO