Fig. 2
ALDOA contributed to G1/S transition in NSCLC. a Flow cytometry analysis of H520 cells after propidium iodide (PI) staining. The percentage of cells in the G0/G1 phase was significantly increased in the shAL group compared with that in shNC group. Data are shown as mean ± standard deviation (SD). *P < 0.05 versus shNC. b The percentage of cells in the G0/G1 phase was significantly decreased in p-AL compared with that in p-NC group. c H520 cells were synchronized by the microtubule depolymerizing agent nocodazole for 20 h, released in RPMI1640 supplemented with 10% fetal bovine serum, and harvested every 2 h thereafter. ALDOA was measured by western blotting. Cyclin B1, cyclin D1, cyclin E1 and cyclin A1 were monitored as indicators of the G2/M, G1, G1/S and S phase, respectively. The protein levels were quantified by Gel-Pro Analyzer (Media Cybernetics, Rockville, MD, US). The experiment was repeated three times, and representative data are shown. The results suggested that the protein levels of ALDOA increased in the G1 phase and G1/S transition. β-actin served as loading control. d Retinoblastoma protein (Rb), phosphorylated Rb (p-Rb) (Ser780), cyclin D1, cyclin E1, cyclin-dependent kinase 4 (CDK4) and CDK6 were tested by western blotting. Cyclin D1, cyclin E1, CDK6 and p-Rb (Ser780) were decreased in shAL H520 cells compared with shNC cells, and CDK4 and Rb remained unchanged. β-actin served as loading control. Data are shown as mean ± SD. *P < 0.05 versus shNC. e p-Rb and cyclin D1 were increased in p-AL cells compared with that in p-NC cells. Rb, CDK4, CDK6 and cyclin E1 remained unchanged. β-actin served as loading control. Data are shown as mean ± SD. *P < 0.05 versus shNC