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Table 1 Primer and probe sequences used in qPCR for TSGs and EBV BamHI-W detection

From: Nasopharyngeal brushing: a convenient and feasible sampling method for nucleic acid-based nasopharyngeal carcinoma research

Primer Sequences Tm (°C) Amplicon length (bp)
CACNA2D3 Forward, 5′-CAGCTGCCTCTGTGAATCTGTGG-3′ 63.58 288
Reverse, 5′-ATGATTTAGCATGCCAAGAGAACATC-3′ 60.24
PRDM5 Forward, 5′-CAGGTTCTCCCTGAAGTCCT-3′ 58.35 249
Reverse, 5′-TGAGATGGTGCCTCATGAAC-3′ 57.59
WIF1 Forward, 5′-TGAATTCCTGTCCTTGCGCT-3′ 59.96 296
Reverse, 5′-ACTCGCAGATGCGTCTTTCA-3′ 60.04
RIZ1 Forward, 5′-TGAATCAGAACACTACTGAGCC-3′ 57.81 297
Reverse, 5′-GCAGCCAGTTTCCCTTCTCT-3′ 59.96
CHFR Forward, 5′-AACCGGAACACAGGTCTGG-3′ 59.55 247
Reverse, 5′-TGGACGGTTTGGGCATTTCT-3′ 60.18
RASFF1A Forward, 5′-CGCGCATTGCAAGTTCACC-3′ 61.08 242
Reverse, 5′-AGCCTGTGTAAGAACCGTCC-3′ 59.68
RPS13 Forward, 5′-TCGGCTTTACCCTATCGACGCAG-3′ 64.47 153
Reverse, 5′-ACGTACTTGTGCAACACCATGTGA-3′ 63.24
BamHI-W Forward, 5′-CCCAACACTCCACCACACC-3′ 60.53 76
Reverse, 5′-TCTTAGGAGCTGTCCGAGGG-3′ 60.40
Hybridization probe 5′-FAMCACACACTACACACACCCACCCGTCTC-TAMRA-3′   
  1. qPCR quantitative polymerase chain reaction, EBV Epstein–Barr virus, CACNA2D3 calcium channel regulatory subunit α2δ3, PRDM5 positive regulatory domain I-binding factor 1 and retinoblastoma protein-interacting zinc-finger gene domain-containing protein 5, WIF1 WNT inhibitory factor 1, RIZ1 retinoblastoma protein-interacting zinc finger gene 1, CHFR checkpoint with forkhead and ring finger domains, RASFF1A Ras association domain family 1A, RPS13 ribosomal protein S13