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Table 1 Primer and probe sequences used in qPCR for TSGs and EBV BamHI-W detection

From: Nasopharyngeal brushing: a convenient and feasible sampling method for nucleic acid-based nasopharyngeal carcinoma research

Primer

Sequences

Tm (°C)

Amplicon length (bp)

CACNA2D3

Forward, 5′-CAGCTGCCTCTGTGAATCTGTGG-3′

63.58

288

Reverse, 5′-ATGATTTAGCATGCCAAGAGAACATC-3′

60.24

PRDM5

Forward, 5′-CAGGTTCTCCCTGAAGTCCT-3′

58.35

249

Reverse, 5′-TGAGATGGTGCCTCATGAAC-3′

57.59

WIF1

Forward, 5′-TGAATTCCTGTCCTTGCGCT-3′

59.96

296

Reverse, 5′-ACTCGCAGATGCGTCTTTCA-3′

60.04

RIZ1

Forward, 5′-TGAATCAGAACACTACTGAGCC-3′

57.81

297

Reverse, 5′-GCAGCCAGTTTCCCTTCTCT-3′

59.96

CHFR

Forward, 5′-AACCGGAACACAGGTCTGG-3′

59.55

247

Reverse, 5′-TGGACGGTTTGGGCATTTCT-3′

60.18

RASFF1A

Forward, 5′-CGCGCATTGCAAGTTCACC-3′

61.08

242

Reverse, 5′-AGCCTGTGTAAGAACCGTCC-3′

59.68

RPS13

Forward, 5′-TCGGCTTTACCCTATCGACGCAG-3′

64.47

153

Reverse, 5′-ACGTACTTGTGCAACACCATGTGA-3′

63.24

BamHI-W

Forward, 5′-CCCAACACTCCACCACACC-3′

60.53

76

Reverse, 5′-TCTTAGGAGCTGTCCGAGGG-3′

60.40

Hybridization probe

5′-FAMCACACACTACACACACCCACCCGTCTC-TAMRA-3′

  
  1. qPCR quantitative polymerase chain reaction, EBV Epstein–Barr virus, CACNA2D3 calcium channel regulatory subunit α2δ3, PRDM5 positive regulatory domain I-binding factor 1 and retinoblastoma protein-interacting zinc-finger gene domain-containing protein 5, WIF1 WNT inhibitory factor 1, RIZ1 retinoblastoma protein-interacting zinc finger gene 1, CHFR checkpoint with forkhead and ring finger domains, RASFF1A Ras association domain family 1A, RPS13 ribosomal protein S13