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Table 1 Oligonucleotides used in the study

From: EYA4 inhibits hepatocellular carcinoma growth and invasion by suppressing NF-κB-dependent RAP1 transactivation

Target genes Sequences Length of product (bp)
shRNA inserts
 EYA4 F: 5′-GATCCCGCACTTAAGTCTTTATCAATTCAAGAGATTGATAAAGACTTAAGTGCTTTTTTCCAAA-3′ 64
R: 5′-AGCTTTTGGAAAAAAGCACTTAAGTCTTTATCAATCTCTTGAATTGATAAAGACTTAAGTGCGG-3′ 64
 Scramble F: 5′-GATCCCGTCTCATTATTGCTAAACAGTTGATATCCGCTGTTTAGCAATAATGAGATTTTTTCCAAA-3′ 66
R: 5′-AGCTTTTGGAAAAAATCTCATTATTGCTAAACAGCGGATATCAACTGTTTAGCAATAATGAGACGG-3′ 66
qRT-PCR
 EYA4 F: 5′-GAATAACACAGCCGATGG-3′ 18
R: 5′-CCAGGTCACTATCAGGAG-3′ 18
 RAP1A F: 5′-CTCCTGAACCAAGGACCACT-3′ 20
R: 5′-TGTGTCTCACTGCACCTTCA-3′ 20
 BCL-2 F: 5′-GAGAAATCAAACAGAGGCCG-3′ 20
R: 5′-CTGAGTACCTGAACCGGCA-3′ 19
 CCND1 F: 5′-GGCGGATTGGAAATGAACTT-3′ 20
R: 5′-TCCTCTCCAAAATGCCAGAG-3′ 20
 TWIST1 F: 5′-TCCATTTTCTCCTTCTCTGGAA-3′ 22
R: 5′-GTCCGCGTCCCACTAGC-3′ 17
 XIAP F: 5′-GACCCTCCCCTTGGACC-3′ 17
R: 5′-CTGTTAAAAGTCATCTTCTCTTGAAA-3′ 26
 GAPDH F: 5′-AATCCCATCACCATCTTCCA-3′ 20
R: 5′-TGGACTCCACGACGTACTCA-3′ 20
ChIP
 RAP1A promoter F: 5′-CATAAGCATTTACCAGGAAAATGG-3′ 24
R: 5′-GGTTCCTAACAGATGATGGAAA-3′ 22
  1. EYA4 eyes absent homolog 4, RAP1A RAS-related protein 1A gene, BCL-2 B cell leukemia/lymphoma 2, CCND1 cyclin D1, TWIST1 twist-related protein 1, XIAP X-linked inhibitor of apoptosis, GAPDH glyceraldehyde 3-phosphate dehydrogenase, shRNA short hairpin RNA, qRT-PCR quantitative real-time polymerase chain reaction, ChIP chromatin immunoprecipitation
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Cancer Communications

ISSN: 2523-3548

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