Table 1 Oligonucleotides used in the study
Target genes | Sequences | Length of product (bp) |
|---|---|---|
shRNA inserts | ||
EYA4 | F: 5′-GATCCCGCACTTAAGTCTTTATCAATTCAAGAGATTGATAAAGACTTAAGTGCTTTTTTCCAAA-3′ | 64 |
R: 5′-AGCTTTTGGAAAAAAGCACTTAAGTCTTTATCAATCTCTTGAATTGATAAAGACTTAAGTGCGG-3′ | 64 | |
Scramble | F: 5′-GATCCCGTCTCATTATTGCTAAACAGTTGATATCCGCTGTTTAGCAATAATGAGATTTTTTCCAAA-3′ | 66 |
R: 5′-AGCTTTTGGAAAAAATCTCATTATTGCTAAACAGCGGATATCAACTGTTTAGCAATAATGAGACGG-3′ | 66 | |
qRT-PCR | ||
EYA4 | F: 5′-GAATAACACAGCCGATGG-3′ | 18 |
R: 5′-CCAGGTCACTATCAGGAG-3′ | 18 | |
RAP1A | F: 5′-CTCCTGAACCAAGGACCACT-3′ | 20 |
R: 5′-TGTGTCTCACTGCACCTTCA-3′ | 20 | |
BCL-2 | F: 5′-GAGAAATCAAACAGAGGCCG-3′ | 20 |
R: 5′-CTGAGTACCTGAACCGGCA-3′ | 19 | |
CCND1 | F: 5′-GGCGGATTGGAAATGAACTT-3′ | 20 |
R: 5′-TCCTCTCCAAAATGCCAGAG-3′ | 20 | |
TWIST1 | F: 5′-TCCATTTTCTCCTTCTCTGGAA-3′ | 22 |
R: 5′-GTCCGCGTCCCACTAGC-3′ | 17 | |
XIAP | F: 5′-GACCCTCCCCTTGGACC-3′ | 17 |
R: 5′-CTGTTAAAAGTCATCTTCTCTTGAAA-3′ | 26 | |
GAPDH | F: 5′-AATCCCATCACCATCTTCCA-3′ | 20 |
R: 5′-TGGACTCCACGACGTACTCA-3′ | 20 | |
ChIP | ||
RAP1A promoter | F: 5′-CATAAGCATTTACCAGGAAAATGG-3′ | 24 |
R: 5′-GGTTCCTAACAGATGATGGAAA-3′ | 22 | |