Fig. 2

EYA4 negatively regulated RAS-related protein 1 (RAP1) expression in human HCC cells. a A heat map of gene expression microarray data shows significantly repressed genes (fold change > 3) in stable pEYA4 transfectants compared with vector transfectants. b Comparison of gene expression in the indicated pEYA4 versus vector cells through microarray profiling. c Venn diagram for the selection of RAS-related protein 1A gene (RAP1A) using vector- and pEYA4-expressing Huh-7 and PLC5 cells. d qRT-PCR analyses of RAP1A and EYA4 mRNA expression in pEYA4 transfectants. **P < 0.01 and ***P < 0.001 versus the vector group. e Western blotting analyses of the RAP1 and EYA4 protein expression levels in pEYA4 transfectants. f Western blotting analyses of RAP1 protein expression levels in the indicated HCC cells after EYA4 depletion and subsequent pEYA4 transfection. g Weight of subcutaneous xenograft tumors formed by pEYA4 and vector transfectants. **P < 0.01 between groups. h Western blotting analyses of the EYA4 and RAP1 protein expression levels in the subcutaneous xenograft tumors formed by pEYA4 and vector transfectants. EYA4 eyes absent homolog 4, GAPDH glyceraldehyde 3-phosphate dehydrogenase, pEYA4 EYA4-expressing plasmid, RAP1 RAS-related protein 1, RAP1A RAS-related protein 1A gene