Table 1 Primers and reaction conditions for the measurement of gene transcriptional levels using quantitative reverse transcription-polymerase chain reaction
From: Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma
Gene | Reaction program | Forward primer (5′–3′) | Reverse primer (5′–3′) | E (%) | R 2 | Location |
|---|---|---|---|---|---|---|
FSTL1 | 95 °C for 3 min; 45 cycles of 95 °C for 10 s, 60 °C for 10 s, and 72 °C for 25 s | AAATGCAGCTCCCTGTCCAA | ACTCTTGCCCTCCTCCCATAG | 95.5 | 0.999 | Transcript: NM_007085.4 Forward primer: exon 11 Reverse primer: exon 11 |
IL-6 | 95 °C for 10 min; 45 cycles of 95 °C for 10 s, 60 °C for 10 s, and 72 °C for 25 s | GCTTTAAGGAGTTCCTGC | GGTAAGCCTACACTTTCCA | 102.6 | 0.997 | Transcript: NM_000600.4 Forward primer: exon 5 Reverse primer: exon 5 |
N-cadherin | 95 °C for 3 min; 45 cycles of 95 °C for 10 s, 60 °C for 10 s, and 72 °C for 25 s | TGGATGAAGATGGCATGG | AGGTGGCCACTGTGCTTAC | 98.6 | 0.999 | Transcript: NM_001792.4 Forward primer: exon 3 Reverse primer: exon 4 |
E-cadherin | 95 °C for 4 min; 40 cycles of 95 °C for 10 s and 60 °C for 45 s | GTCATCCAACGGGAATGCA | TGATCGGTTACCGTGATCAAAA | 97.1 | 0.999 | Transcript: NM_004360.4 Forward primer: exon 4 Reverse primer: exon 5 |
GAPDH | 95 °C for 10 min; 45 cycles of 95 °C for 10 s, 60 °C for 10 s, and 72 °C for 25 s | TGACTTCAACAGCGACACCCA | CACCCTGTTGCTGTAGCCAAA | 101.5 | 0.998 | Transcript: NM_002046.5 Forward primer: exon 6 Reverse primer: exon 4 |