Fig. 3
From: Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma
Effect of FSTL1 knockdown on cell cycle and adhesion molecule expression in ccRCC cells. a Effect of FSTL1 knockdown on cell cycle progression of NRCC cells. The percentage of cells in S and G2/M phases is higher in NRCC-shFSTL1 cells (55.4% ± 4.0% and 8.8% ± 1.4%) than in NRCC-shScramble cells (39.0% ± 4.2% and 1.5% ± 0.8%). The first and second peaks of the red curve represent the cell count of G0/G1 and G2 phases, respectively. The shaded plot reflects the cell count of S phase. b, c Effect of FSTL1 knockdown on the expression of CD99 and CD24 on NRCC cells. The percentage of CD99-positive cells is higher in NRCC-shFSTL1 cells (44.7% ± 4.3%) than in NRCC-shScramble cells (15.3% ± 1.5%), whereas the percentage of CD24-positive cells is lower in NRCC-shFSTL1 cells than in NRCC-shScramble cells (55.5% ± 6.8% vs. 85.0% ± 1.2%). shFSTL1, FSTL1 knockdown; shScramle, vector control. Each flow cytometry assay was conducted in triplicate and a representative figure is shown