Fig. 3

Promotion of Huh7 and Hep3B cell growth by TRIM3 silencing. a, b Proliferation assay results show that silencing TRIM3 expression promoted proliferation of Huh7 (a) and Hep3B (b) cells. c, d Left representative images of increased colony formation in a monolayer culture by silencing TRIM3. Right colony-forming efficiencies were calculated after 12-day conventional culture. Measurements were carried out in triplicate, and experiments were repeated three times. Data are presented as mean ± SD. P values were calculated using Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001, versus cells transfected with siNC