Table 2 Primer sequences for quantitative real-time PCR
Gene | Forward sequence | Reverse sequence | Product length (bp) |
|---|---|---|---|
RNF183 | 5′-CGAAAAGCTTGAAGGACTGG-3′ | 5′-TGAAGCAGCTCCAGTGAGAA-3′ | 166 |
SELE | 5′-GGACACAGCAAATCCCAGTT-3′ | 5′-CTCCAATAGGGGAATGAGCA-3′ | 266 |
ICAM1 | 5′-GAGATCACCATGGAGCCAAT-3′ | 5′-CTGACAAGTTGTGGGGGAGT-3′ | 120 |
MMP9 | 5′-TTGACAGCGACAAGAAGTGG-3′ | 5′-CCCTCAGTGAAGCGGTACAT-3′ | 129 |
PLAU | 5′-GCCATCCCGGACTATACAGA-3′ | 5′-ACACAGCATTTTGGTGGTGA-3′ | 195 |
CXCR4 | 5′-TGAGAAGCATGACGGACAAG-3′ | 5′-GACGCCAACATAGACCACCT-3′ | 156 |
IL-8 | 5′-CTGCGCCAACACAGAAATTAT-3′ | 5′-CATCTGGCAACCCTACAACAG-3′ | 214 |
IL-6 | 5′-TCAATGAGGAGACTTGCCTGGTGA-3′ | 5′-TCATCTGCACAGCTCTGGCTTGTT-3′ | 120 |
GAPDH | 5′-CTCCTCCTGTTCGACAGTCAGC-3′ | 5′-CCCAATACGACCAAATCCGTT-3′ | 185 |