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Table 2 Primer sequences for quantitative real-time PCR

From: Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cells

Gene Forward sequence Reverse sequence Product length (bp)
RNF183 5′-CGAAAAGCTTGAAGGACTGG-3′ 5′-TGAAGCAGCTCCAGTGAGAA-3′ 166
SELE 5′-GGACACAGCAAATCCCAGTT-3′ 5′-CTCCAATAGGGGAATGAGCA-3′ 266
ICAM1 5′-GAGATCACCATGGAGCCAAT-3′ 5′-CTGACAAGTTGTGGGGGAGT-3′ 120
MMP9 5′-TTGACAGCGACAAGAAGTGG-3′ 5′-CCCTCAGTGAAGCGGTACAT-3′ 129
PLAU 5′-GCCATCCCGGACTATACAGA-3′ 5′-ACACAGCATTTTGGTGGTGA-3′ 195
CXCR4 5′-TGAGAAGCATGACGGACAAG-3′ 5′-GACGCCAACATAGACCACCT-3′ 156
IL-8 5′-CTGCGCCAACACAGAAATTAT-3′ 5′-CATCTGGCAACCCTACAACAG-3′ 214
IL-6 5′-TCAATGAGGAGACTTGCCTGGTGA-3′ 5′-TCATCTGCACAGCTCTGGCTTGTT-3′ 120
GAPDH 5′-CTCCTCCTGTTCGACAGTCAGC-3′ 5′-CCCAATACGACCAAATCCGTT-3′ 185
  1. RNF183 ring finger protein 183, SELE selectin E, ICAM1 intercellular adhesion molecule 1, MMP9 matrix metallo peptidase 9, PLAU plasminogen activator urokinase, CXCR4 C-X-C motif chemokine receptor 4, IL-8 interleukin-8, IL-6 interleukin-6, GAPDH glyceraldehyde-3-phosphate dehydrogenase