Fig. 4From: Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cellsSynergistic antitumor effects of RNF183 knockdown and trametinib treatment on xenograft tumor growth of HCT116 cells. a A representative image of tumors collected from mice inoculated with HCT116 cells that were stably transfected with shRNF183 or shNC and treated with or without trametinib. When tumor reached 100 mm3, 6 mice respectively from the shRNF183 or shNC groups were given 300 μg/kg trametinib every day for 23 days. The remaining 6 mice in each group were treated with vehicle as control. b RNF183 knockdown and trametinib synergistically reduced tumor volumes (i) and tumor weights (ii). c qPCR assay results show that the IL-8 levels in xenograft tumors were synergistically repressed by RNF183 knockdown and trametinib treatment. d Images exhibiting the hematoxylin–eosin (H&E), RNF183, and Ki-67 staining for xenograft tumors, which indicate decreased intensity of Ki-67 in RNF183-knockdown, trametinib treatment, and combination treatment groups. RNF183 is located in the cytoplasm and nucleus and Ki-67 is located in the nucleus. ***P < 0.001Back to article page