Fig. 1From: Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cellsSynthetic lethal short hairpin RNA (shRNA) screening shows that ring finger protein 183 (RNF183) confers resistance to trametinib treatment in colorectal cancer (CRC) cells. a HT29 cells were infected with a pooled shRNA library, and the puromycin-selected stable cells were treated with dimethyl sulfoxide (DMSO) or 20 nmol/L trametinib for 8 days. Then, shRNA sequences were amplified by PCR, and next-generation sequencing was conducted to calculate their abundance. b The abundance of RNF183 shRNA decreased in cells treated with trametinib compared with those treated with DMSO. T/D ratio of abundance, the ratio of abundance in the trametinib group to abundance in the DMSO groupBack to article page