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Table 1 Primers for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR

From: Down-regulation of E-cadherin enhances prostate cancer chemoresistance via Notch signaling

Name Forward sequence Reverse sequence Product length (bp)
E-cadherin 5′-CGGGAATGCAGTTGAGGATC-3′ 5′-AGGATGGTGTAAGCGATGGC-3′ 201
Snail 5′-GAAAGGCCTTCAACTGCAAA-3′ 5′-TGACATCTGAGTGGGTCTGG-3′ 249
N-cadherin 5′-AGCCTGGAACATATGTGATGA-3′ 5′-CCATAAAACGTCATGGCAGTAA-3′ 325
Vimentin 5′-GACAATGCGTCTCTGGCACGTCTT-3′ 5′-TCCTCCGCCTCCTGCAGGTTCTT-3′ 236
ZEB-1 5′-TTCAAACCCATAGTGGTTGCT-3′ 5′-TGGGAGATACCAAACCAACTG-3′ 151
Twist 5′-GGAGTCCGCAGTCTTACGAG-3′ 5′-TCTGGAGGACCTGGTAGAGG-3′ 201
Smad3 5′-ACCAGGGCTTTGAGGCTGTC-3′ 5′-GCAAAGGCCCATTCAGGTG-3′ 144
TGF-β 5′-AGGGCTACCATGCCAACTTC-3′ 5′-CCACGTAGTAGACGATGGGC-3′ 168
β-Catenin 5′-GCTGCACAGGTGACCACATT-3′ 5′-GAGTGCTGAAGGTGCTGTCTGT-3′ 238
Notch-1 5′-GGCACTTTCTGTGAGGAGGA-3′ 5′-GCAGTCAGGCGTGTTGTTCT-3′ 147
GAPDH 5′-AGCCACATCGCTCAGACA-3′ 5′-GCCCAATACGACCAAATCC-3′ 66
  1. ZEB-1 E-box binding homeobox-1, TGF-β transforming growth factor beta, GAPDH glyceraldehyde-3-phosphate dehydrogenase, bp base pair