Table 1 Primers for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR
From: Down-regulation of E-cadherin enhances prostate cancer chemoresistance via Notch signaling
Name | Forward sequence | Reverse sequence | Product length (bp) |
|---|---|---|---|
E-cadherin | 5′-CGGGAATGCAGTTGAGGATC-3′ | 5′-AGGATGGTGTAAGCGATGGC-3′ | 201 |
Snail | 5′-GAAAGGCCTTCAACTGCAAA-3′ | 5′-TGACATCTGAGTGGGTCTGG-3′ | 249 |
N-cadherin | 5′-AGCCTGGAACATATGTGATGA-3′ | 5′-CCATAAAACGTCATGGCAGTAA-3′ | 325 |
Vimentin | 5′-GACAATGCGTCTCTGGCACGTCTT-3′ | 5′-TCCTCCGCCTCCTGCAGGTTCTT-3′ | 236 |
ZEB-1 | 5′-TTCAAACCCATAGTGGTTGCT-3′ | 5′-TGGGAGATACCAAACCAACTG-3′ | 151 |
Twist | 5′-GGAGTCCGCAGTCTTACGAG-3′ | 5′-TCTGGAGGACCTGGTAGAGG-3′ | 201 |
Smad3 | 5′-ACCAGGGCTTTGAGGCTGTC-3′ | 5′-GCAAAGGCCCATTCAGGTG-3′ | 144 |
TGF-β | 5′-AGGGCTACCATGCCAACTTC-3′ | 5′-CCACGTAGTAGACGATGGGC-3′ | 168 |
β-Catenin | 5′-GCTGCACAGGTGACCACATT-3′ | 5′-GAGTGCTGAAGGTGCTGTCTGT-3′ | 238 |
Notch-1 | 5′-GGCACTTTCTGTGAGGAGGA-3′ | 5′-GCAGTCAGGCGTGTTGTTCT-3′ | 147 |
GAPDH | 5′-AGCCACATCGCTCAGACA-3′ | 5′-GCCCAATACGACCAAATCC-3′ | 66 |