Fig. 5

Silencing E-cadherin expression in PC3 and DU145 cells causes EMT-mediated paclitaxel tolerance. a qPCR (top) and Western blotting analysis (bottom) of E-cadherin mRNA and protein expression in PC3 and DU145 cell lines after small interfering RNA (siRNA)-mediated E-cadherin silencing. Cells transfected with negative control siRNA are named PC3-nc and DU145-nc, respectively; cells transfected with E-cadherin siRNA are named PC3-si-E-cadherin-1, PC3-si-E-cadherin-2, DU145-si-E-cadherin-1, and DU145-si-E-cadherin-2. b Expression of mesenchymal markers Snail, Vimentin, and N-cadherin were measured using qPCR. c Wounded DU145, DU145-nc, and DU145-si-E-cadherin-1 cell monolayers were photographed 0, 24, and 36 h after the mechanical scratch, and the width of the wounds was measured in 3 independent wound sites per group. d Colony formation abilities of parental cells, negative control cells, and E-cadherin-silencing cells were tested. The numbers of colonies are shown. e Silencing E-cadherin expression induces the resistance of PCa cells to paclitaxel. Cells were treated with paclitaxel (range: 0–80 nmol/L) for 72 h. Cell survival was determined using MTS assay. The mean ± SEM values of data from three independent experiments are presented. **P < 0.01, *P < 0.05