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Fig. 2 | Chinese Journal of Cancer

Fig. 2

From: The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells

Fig. 2

ASB3 inhibits the proliferation of CRC cells in vitro. ASB3 protein levels, cell proliferation, or cell cycle distributions were analyzed in CRC cells using Western blotting assay (a and e), MTT assay (b and c), colony formation assay (d and f), or flow cytometry (g) after ASB3 knockdown by transiently transfected with small interfering RNAs (siRNAs) or after ASB3 overexpression by stably transfected with ASB3 cDNA or its mutants. siNC, negative control siRNA; WT, wild type; G135E, K339I, R362C, and ΔSOCS are ASB3 mutants. Representative pictures are also included in d, f, and g. *P < 0.05 compared with those transfected with siNC or vector control; P < 0.05 compared with that transfected with WT ASB3; ***P < 0.001 compared with that transfected with siNC

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