Fig. 1

Different tumor morphology, histologic features, and LOH patterns of CHC and SHC. CHC is defined as a tumor with an intimate admixture of both HCC and ICC components, whereas SHC is defined as two separated HCC and ICC nodules arise in the same liver. Case 5 a shows no LOH in NT, HCC, and ICC for marker D8S258 (four bands presented at the same position). Case 4 b shows LOH in HCC for marker D4S406 (a band was lost in HCC compared with that in NT). Left, morphology; middle, histology; right, LOH pattern. Dotted line in a indicates the boundary between HCC and ICC components; yellow arrow in b indicates a reduction in density of >50% compared with paracancerous tissue. NT corresponding non-tumorous tissue, CHC combined hepatocellular and cholangiocarcinoma, SHC separated hepatocellular carcinoma and intrahepatic cholangiocarcinoma, LOH loss of heterozygosity, HCC hepatocellular carcinoma, ICC intrahepatic cholangiocarcinoma