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Fig. 1 | Chinese Journal of Cancer

Fig. 1

From: EYA4 gene functions as a prognostic marker and inhibits the growth of intrahepatic cholangiocarcinoma

Fig. 1

Eyes absent homolog 4 (EYA4) mRNA and protein expression in intrahepatic cholangiocarcinoma (ICC) tissues. a Boxplot of EYA4 real-time quantitative polymerase chain reaction shows that ICC tissues have lower EYA4 mRNA levels than non-tumorous adjacent liver tissues (*P < 0.001). ΔCt stands for difference in cycle threshold; it was calculated as follows: ΔCt = CtTarget gene − CtGAPDH. b Positive control staining of human skeleton muscle tissue using EYA4 antibody. c Negative control staining of human skeleton muscle using rabbit IgG as the primary antibody. d Boxplot of EYA4 immunohistochemical (IHC) scores shows that ICC tissues have lower EYA4 protein level than non-tumorous adjacent liver tissues (*P < 0.001). e A typical staining of adjacent non-tumorous liver tissue. f A weak staining in tumor tissues with lymph node metastasis. g A strong staining in tumor tissues without lymph node metastasis. Black arrows indicate positive staining of hepatocytes and ICC cells. L liver. T tumor. M muscle. h Boxplot of EYA4 IHC scores shows that multiple tumors have lower EYA4 protein levels than single tumors (*P = 0.023). i Boxplot of EYA4 IHC scores shows that tumor with lymph node metastasis have lower EYA4 protein levels than those without lymph node metastasis (*P < 0.001)

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