Fig. 2

Pathological and cytological findings in an SCLC patient with LEMS. Specimens were obtained by cervical lymph node biopsy on February 14, 2002. Paraffin-embedded sections were stained with antibody using the polymer method (The Dako Envision™ FLEX, Dako Japan, Tokyo, Japan) and signal was developed with diaminobenzidine (DAB). Sections were counterstained with hematoxylin. Positive staining for markers in cytoplasm and on cell membrane was indicated in brown. a There are dense sheets of small cells with nuclear molding and necrosis (hematoxylin–eosin staining). b CD56 was diffusely and strongly positive on cell membrane. c Chromogranin A was diffusely and moderately expressed in cytoplasm. d Synaptophysin was focally and weakly expressed in cytoplasm