Fig. 4

Overexpression of CEP78 in CRC cells inhibits cell viability in vitro. a Expression of CEP78 in CRC cell lines was determined by western blotting. b CEP78 protein levels were examined by western blotting in cells with or without CEP78 overexpression. c MTT assay results showed a significant reduction of viability in cells with CEP78 overexpression. d Colony formation was decreased in cells with CEP78 overexpression. The results of colony assay quantification are indicated as the mean ± standard error of the mean (SEM) of three independent experiments. *P < 0.05. e Cells were transfected with CEP78 or empty vector for 48 h. The distributions of cell cycle were determined by flow cytometry analyses. f Cells treated as described in e were subjected to apoptosis analyses, using Annexin V/PI staining